Fly-tippers and bin rummagers are being warned to clean up their act or face being fined or taken to court.
Local residents are also being urged to follow the rules when leaving out waste and recycling for collections.
The call follows ongoing issues in a number back alleys which are delaying crews, creating environmental and health hazards and wasting much-needed resources.
Although some services to have been put on hold during the response to the Coronavirus pandemic, household refuse, recycling and garden waste collections are up and running.
Residents are reminded to:
- Place all waste in the bins provided
- Bag waste rather than place it loose in the bin or on the ground
- Ensure back yards are kept clean and no build of waste as this can attract rodents
Large items should not be placed in rear alleys - while the bulky waste services remains suspended, residents can book online to dispose of large items at the Household Waste and Recycling Centre at Haverton Hill.
Those without access to a car are asked to keep any such items on their property until the bulky waste service resumes.
The town's extensive CCTV network will be used to identify those fly-tipping and rummaging through bins, with enforcement action taken where possible.
Middlesbrough Mayor Andy Preston said: "I'm enormously grateful to local residents for the patience and understanding they have shown during this unprecedented period.
"However there is a small minority who are ignoring what are perfectly reasonable rules, and that can make life a misery for their neighbours, and for our hard-working crews.
"If we all work together with thought and consideration we can keep our alleys and other public areas spick and span, and we hope to have things back to normal as soon as possible."
Anyone witnessing anti-social behaviour or people going through bins is urged to report it immediately by calling 01642 726001 or visiting the Government website
For the latest information on waste and recycling collections in Middlesbrough, visit the recycling and rubbish page
For the latest local updates and information, visit the Council's COVID-19 page
For information on local help and support, visit Help Boro or call Middlesbrough Council's support line 01642 729777